本文通过计算序列分析预测split intein的聚合倾向，发现LCGC14新型高效无半胱氨酸split intein，并与CLm正交，实现三段蛋白串联连接及选择性化学标记，拓展了蛋白质工程应用范围。 这篇文章介绍了一种利用分裂intein（split intein）进行蛋白质工程的新方法，特别是在 ...

The alternative text for this image may have been generated using AI. Here we demonstrate that protein splicing could also occur as a bimolecular reaction involving cis-splicing precursors, thereby ...

Protein splicing is a self-catalysed post-translational process in which an intervening polypeptide segment, known as an intein, excises itself from a precursor protein and ligates the flanking ...

Why do problems occur with a special variant of 'protein glues', the split inteins, that severely limit their use in producing proteins? A team has now answered this question. Proteins are the ...

The SCN1A gene is too large to fit into aconventional viral delivery systems. Scientists overcame this hurdle with a pioneering "split-intein" mechanism, which is like two separate trucks, delivering ...